pLO87
(Plasmid
#91726)
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Purposemycobacterial expression of mCherry from Hsp60 promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 91726 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLO86
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Backbone manufacturerHatfull lab, Addgene plasmid 91724
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Vector typemycobacterial expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehsp60 promoter
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SpeciesM. bovis
- Promoter hsp60
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Tag
/ Fusion Protein
- mCherry (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLO87 was a gift from Graham Hatfull (Addgene plasmid # 91726 ; http://n2t.net/addgene:91726 ; RRID:Addgene_91726) -
For your References section:
Mutational analysis of the mycobacteriophage BPs promoter PR reveals context-dependent sequences for mycobacterial gene expression. Oldfield LM, Hatfull GF. J Bacteriol. 2014 Oct;196(20):3589-97. doi: 10.1128/JB.01801-14. Epub 2014 Aug 4. 10.1128/JB.01801-14 PubMed 25092027
