pST39-pNL29
(Plasmid #91696)

• Purpose: Expresses Mega GVs in E.Coli
• Depositing Lab: Mikhail Shapiro
• Publication: Lakshmanan et al Nat Protoc. 2017 Oct;12(10):2050-2080. doi: 10.1038/nprot.2017.081. Epub 2017 Sep 7.

Backbone

• Vector backbone: pST39
• Backbone manufacturer: Tan Lab
• Backbone size w/o insert (bp): 3119
• Total vector size (bp): 8765
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mega GV gene cluster (from pNL29) in pST39 backbone
• Alt name: Mega GV gene cluster
• Species: Bacillus Megaterium
• Insert Size (bp): 5646
• GenBank ID: AF053765
• Promoter: T7
• Tag / Fusion Protein:
  • No tags

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• Supplemental Documents:
  • pST39-pNL29_Addgene.gb
• A portion of this plasmid was derived from a plasmid made by: Li, N. and Cannon, M.C. Gas Vesicle Genes Identified in Bacillus megaterium and Functional Expression in Escherichia coli. J Bacteriol. 1998 May; 180(9): 2450–2458.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry