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Addgene

pSY6
(Plasmid #90482)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 90482 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIMP1-ptb
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    group II intron and ItrA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

E. coli/Clostridium shuttle vector, derived from pIMP1-Pptb by inserting the groupII intron and ItrA ORF from pMTL20lacZT-TErmBtdRAM2

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSY6 was a gift from Sheng Yang (Addgene plasmid # 90482 ; http://n2t.net/addgene:90482 ; RRID:Addgene_90482)

  • For your References section:

    Targeted gene disruption by use of a group II intron (targetron) vector in Clostridium acetobutylicum. Shao L, Hu S, Yang Y, Gu Y, Chen J, Yang Y, Jiang W, Yang S. Cell Res. 2007 Nov;17(11):963-5. doi: 10.1038/cr.2007.91. 10.1038/cr.2007.91 PubMed 17971808
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