Arf6 T27N- Venus in pcDNA3.1
(Plasmid
#90464)
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PurposeRhoA mediated cell migration
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 90464 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5428
- Total vector size (bp) 6667
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameArf6 T27N
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Insert Size (bp)528
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Entrez GeneARF6
- Promoter CMV
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Tag
/ Fusion Protein
- Venus (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site BglII (destroyed during cloning)
- 5′ sequencing primer T7 forward primer
- 3′ sequencing primer BGH reverse primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The entire Arf6 T27N-Venus can be cut out from pcDNA3.1 using EcoR1 and XbaI
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Arf6 T27N- Venus in pcDNA3.1 was a gift from Narasimhan Gautam (Addgene plasmid # 90464 ; http://n2t.net/addgene:90464 ; RRID:Addgene_90464) -
For your References section:
SRRF-Stream Imaging of Optogenetically Controlled Furrow Formation Shows Localized and Coordinated Endocytosis and Exocytosis Mediating Membrane Remodeling. Castillo-Badillo JA, Bandi AC, Harlalka S, Gautam N. ACS Synth Biol. 2020 Mar 16. doi: 10.1021/acssynbio.9b00521. 10.1021/acssynbio.9b00521 PubMed 32155337