pLKO5d.SFFV.dCas9-KRAB.P2A.BSD
(Plasmid #90332)

• Purpose: CRISPR interference
• Depositing Lab: Dirk Heckl
• Publication: Schwarzer et al Nat Commun. 2017 Aug 9;8(1):218. doi: 10.1038/s41467-017-00212-4.

Backbone

• Vector backbone: pLKO5d
• Total vector size (bp): 7408
• Modifications to backbone: modified pLKO.005
• Vector type: Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: dCas9
• Species: S. pyogenes
• Insert Size (bp): 4500
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Gene/Insert 2

• Gene/Insert name: na

Gene/Insert 3

• Gene/Insert name: SFFV
• Alt name: Spleen Focus Forming Virus Promoter Enhancer
• Species: H. sapiens (human)
• Insert Size (bp): 450

Gene/Insert 4

• Gene/Insert name: P2A-BSD
• Alt name: Blasticidin-S-Deaminase
• Species: Synthetic
• Insert Size (bp): 450

Resource Information

• Supplemental Documents:
  • pLKO5d.SFFV.dCas9-KRAB.P2A.BSD.gb
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry