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PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutation
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 90172 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCIneo
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Backbone manufacturerpromega
- Backbone size w/o insert (bp) 5472
- Total vector size (bp) 7860
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFLuc EGFP
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Speciesfirefly
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Insert Size (bp)2373
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MutationR188Q, R261Q in FLuc
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCI-neo FlucDM EGFP was a gift from Franz-Ulrich Hartl (Addgene plasmid # 90172 ; http://n2t.net/addgene:90172 ; RRID:Addgene_90172) -
For your References section:
Firefly luciferase mutants as sensors of proteome stress. Gupta R, Kasturi P, Bracher A, Loew C, Zheng M, Villella A, Garza D, Hartl FU, Raychaudhuri S. Nat Methods. 2011 Sep 4;8(10):879-84. doi: 10.1038/nmeth.1697. 10.1038/nmeth.1697 PubMed 21892152
