pCI-neo Fluc EGFP
(Plasmid #90170)

• Purpose: EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins
• Depositing Lab: Franz-Ulrich Hartl
• Publication: Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. doi: 10.1038/nmeth.1697.

Backbone

• Vector backbone: pCIneo
• Backbone manufacturer: promega
• Backbone size w/o insert (bp): 5472
• Total vector size (bp): 7860
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: FLuc EGFP
• Species: firefly
• Insert Size (bp): 2373

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XmaI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry