pFcpB-DEST
(Plasmid
#90098)
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Purpose(Empty Backbone) Gateway destination vector featuring FcpB promoter in front of AttR1/2 sites on episomal vector maintained in the diatom Phaeodactylum tricornutum.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 90098 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPtPBR1
- Backbone size (bp) 10370
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Modifications to backbonepPtPBR1 (episomal maintenance in the diatom P. tricornutum) was modified to include FcpB promoter, (AttR1-CatR-ccdB-AttR2), and FcpA terminator.
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Vector typeDestination vector for Gateway expression
- Promoter FcpB
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, Ampicillin, Tetracycline
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFcpB-DEST was a gift from Philip Weyman (Addgene plasmid # 90098 ; http://n2t.net/addgene:90098 ; RRID:Addgene_90098) -
For your References section:
Episomal tools for RNAi in the diatom Phaeodactylum tricornutum. Bielinski VA, Bolt TM, Dupont CL, Weyman PD. PeerJ Preprints. 2017 5:e2907v1 10.7287/peerj.preprints.2907v1
Map uploaded by the depositor.
