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Addgene

pET21b-Caspase-3(D3A,V266E)
(Plasmid #90091)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 90091 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Caspase 3
  • Species
    H. sapiens (human)
  • Mutation
    D175A D28A D9A V266E
  • Entrez Gene
    CASP3 (a.k.a. CPP32, CPP32B, SCA-1)
  • Tag / Fusion Protein
    • His tag (C terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21b-Caspase-3(D3A,V266E) was a gift from Clay Clark (Addgene plasmid # 90091 ; http://n2t.net/addgene:90091 ; RRID:Addgene_90091)

  • For your References section:

    Mutations in the procaspase-3 dimer interface affect the activity of the zymogen. Pop C, Feeney B, Tripathy A, Clark AC. Biochemistry. 2003 Oct 28;42(42):12311-20. 10.1021/bi034999p PubMed 14567692
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