pET21b-Caspase-3(D3A: D175A D28A D9A)
(Plasmid
#90090)
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PurposeExpresses caspase 3 D3A mutant (D175A D28A D9A) in bacterial cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 90090 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET21b
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCaspase 3
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SpeciesH. sapiens (human)
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MutationD175A D28A D9A
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Entrez GeneCASP3 (a.k.a. CPP32, CPP32B, SCA-1)
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Tag
/ Fusion Protein
- His tag (C terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET21b-Caspase-3(D3A: D175A D28A D9A) was a gift from Clay Clark (Addgene plasmid # 90090 ; http://n2t.net/addgene:90090 ; RRID:Addgene_90090) -
For your References section:
An uncleavable procaspase-3 mutant has a lower catalytic efficiency but an active site similar to that of mature caspase-3. Bose K, Pop C, Feeney B, Clark AC. Biochemistry. 2003 Oct 28;42(42):12298-310. 10.1021/bi034998x PubMed 14567691
