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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 11821 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET-23 b
- Backbone size w/o insert (bp) 3666
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCaspase 3
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SpeciesH. sapiens (human)
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Insert Size (bp)999
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Entrez GeneCASP3 (a.k.a. CPP32, CPP32B, SCA-1)
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Tag
/ Fusion Protein
- 6xHis (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminal primer (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET23b-Casp3-His was a gift from Guy Salvesen (Addgene plasmid # 11821 ; http://n2t.net/addgene:11821 ; RRID:Addgene_11821) -
For your References section:
Target protease specificity of the viral serpin CrmA. Analysis of five caspases. Zhou Q, Snipas S, Orth K, Muzio M, Dixit VM, Salvesen GS. J Biol Chem. 1997 Mar 21. 272(12):7797-800. 10.1074/jbc.272.12.7797 PubMed 9065443