Capping Protein
(Plasmid #89950)

• Purpose: F-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends.
• Depositing Labs: Antoine Jégou, Guillaume Romet-Lemonne
• Publication: Shekhar et al Nat Commun. 2015 Nov 13;6:8730. doi: 10.1038/ncomms9730.

Backbone

• Vector backbone: pRSFDuet-1
• Total vector size (bp): 5540
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Use BL21 DE3 Star for protein production
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: F-actin-capping protein subunit alpha-1
• Alt name: CapZ alpha-1
• Species: M. musculus (mouse)
• Insert Size (bp): 861
• Mutation: V222I
• Entrez Gene: Capza1 (a.k.a. CAPZ, CAZ1, Cappa1, capZ alpha-1)
• Tag / Fusion Protein:
  • 6*His (N terminal on insert)

Gene/Insert 2

• Gene/Insert name: F-actin-capping protein subunit beta
• Alt name: CapZ beta
• Species: M. musculus (mouse)
• Insert Size (bp): 819
• Entrez Gene: Capzb (a.k.a. 1700120C01Rik, AI325129, CPB, CPB1, CPB2, CPbeat2, CPbet, CPbeta1, CPbeta2, Cap, Cappb1)

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry