pKH3-avian RSK1 K112/464R
(Plasmid
#8981)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8981 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepKH3
- Backbone size w/o insert (bp) 4800
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameRSK
-
SpeciesG. gallus (chicken)
-
MutationK112R, K464R
-
Entrez GeneRPS6KA1 (a.k.a. RPS6KA, RSK)
-
Tag
/ Fusion Protein
- 3x HA (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See author's map for Addgene plasmid 8998 (pKH3-avian RSK1).
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pKH3-avian RSK1 K112/464R was a gift from John Blenis (Addgene plasmid # 8981 ; http://n2t.net/addgene:8981 ; RRID:Addgene_8981) -
For your References section:
Phosphorylation of p90 ribosomal S6 kinase (RSK) regulates extracellular signal-regulated kinase docking and RSK activity. Roux PP, Richards SA, Blenis J. Mol Cell Biol. 2003 Jul . 23(14):4796-804. 10.1128/MCB.23.14.4796-4804.2003 PubMed 12832467