Skip to main content
Addgene

gamma Synuclein-VC
(Plasmid #89734)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89734 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5213
  • Total vector size (bp) 6068
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SNCG
  • Alt name
    gamma Synuclein (human), BCSG1, SR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    855
  • GenBank ID
    NM_003087
  • Entrez Gene
    SNCG (a.k.a. BCSG1, SR)
  • Promoter CMV
  • Tag / Fusion Protein
    • C-terminal part of Venus (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AflII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer bGH
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Original clone (alpha-Synuclein-VC) comes from Pamela J.McLean.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Original clone (alpha Synuclein-VC) includes already the C-terminal part of venus, and was a gift from Pamela J.McLean.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    gamma Synuclein-VC was a gift from Tiago Outeiro (Addgene plasmid # 89734 ; http://n2t.net/addgene:89734 ; RRID:Addgene_89734)
  • For your References section:

    Dimerization propensities of Synucleins are not predictive for Synuclein aggregation. Eckermann K, Kugler S, Bahr M. Biochim Biophys Acta. 2015 Aug;1852(8):1658-64. doi: 10.1016/j.bbadis.2015.05.002. Epub 2015 May 8. 10.1016/j.bbadis.2015.05.002 PubMed 25960149