VN-gamma Synuclein
(Plasmid #89733)

• Purpose: expresses human gamma Synuclein fused to the N-terminal part of Venus
• Depositing Lab: Tiago Outeiro
• Publication: Eckermann et al Biochim Biophys Acta. 2015 Aug;1852(8):1658-64. doi: 10.1016/j.bbadis.2015.05.002. Epub 2015 May 8.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5472
• Total vector size (bp): 6327
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SNCG
• Alt name: gamma Synuclein (human), BCSG1, SR
• Species: H. sapiens (human)
• Insert Size (bp): 855
• GenBank ID: NM_003087
• Entrez Gene: SNCG (a.k.a. BCSG1, SR)
• Promoter: CMV
• Tag / Fusion Protein:
  • N-terminal part of Venus (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AflII (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: bGH

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Original clone (VN-alpha-Synuclein) comes from Pamela J.McLean.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Original clone (VN-alpha Synuclein) includes already the N-terminal part of venus, and was a gift from Pamela J.McLean.