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Addgene

pCDF-casBCDE(+6)
(Plasmid #89728)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89728 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDF-casBCDE
  • Backbone manufacturer
    PMID:26013814
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Streptomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRISPR array
  • Alt name
    CRISPR mini-cassette
  • Mutation
    Derivative CRISPR array containing a longer version of the wt spacer (+6) extended by 6 nucleotides at the leader-distal end.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site AvrII (not destroyed)
  • 5′ sequencing primer Unknown
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDF-casBCDE(+6) was a gift from Konstantin Kuznedelov & Konstantin Severinov (Addgene plasmid # 89728 ; http://n2t.net/addgene:89728 ; RRID:Addgene_89728)

  • For your References section:

    Altered stoichiometry Escherichia coli Cascade complexes with shortened CRISPR RNA spacers are capable of interference and primed adaptation. Kuznedelov K, Mekler V, Lemak S, Tokmina-Lukaszewska M, Datsenko KA, Jain I, Savitskaya E, Mallon J, Shmakov S, Bothner B, Bailey S, Yakunin AF, Severinov K, Semenova E. Nucleic Acids Res. 2016 Dec 15;44(22):10849-10861. Epub 2016 Oct 13. 10.1093/nar/gkw914 PubMed 27738137
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