His8-TEV-PseH
(Plasmid #89725)

• Purpose: Expresses PseH from C. jejuni with an N-terminal 2xHis Gln 5x His feature, followed by the TEV cleavage site
• Depositing Lab: Barbara Imperiali
• Publication: Zamora et al Methods Enzymol. 2017;597:145-186. doi: 10.1016/bs.mie.2017.06.003. Epub 2017 Jul 5.

Backbone

• Vector backbone: pET30b (+)
• Backbone manufacturer: EMB Biosciences
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 5874
• Modifications to backbone: Incorporation of N-terminal OctaHis tag and TEV cleavage site before insert
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PseH
• Species: Campylobacter jejuni
• Insert Size (bp): 474
• GenBank ID: 905605
• Entrez Gene: pseH (a.k.a. Cj1313)
• Tags / Fusion Proteins:
  • 2xHis Gln 5xHis (N terminal on backbone)
  • TEV (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: TTGATAAAACTTAAAAATTTCGCAGAACTTAATTCTCAAGAA
• 3′ sequencing primer: TTAGCTAGGCAAGGCTTTGCAGTGAGATTGCTTTAAGC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry