pet151D-TOPO BROX 1-411;
(Plasmid
#89602)
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PurposeExpresses full length human Brox in bacteria; internal ID WISP11-297
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 89602 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepet151D-TOPO
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Backbone manufacturerInvitrogen
- Total vector size (bp) 6996
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBROX
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SpeciesH. sapiens (human)
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Insert Size (bp)1236
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Entrez GeneBROX (a.k.a. C1orf58)
- Promoter T7
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Tag
/ Fusion Protein
- His Tag, V5 tag (N terminal on backbone)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer T7 forward
- 3′ sequencing primer T7 term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pet151D-TOPO BROX 1-411; was a gift from Wesley Sundquist (Addgene plasmid # 89602 ; http://n2t.net/addgene:89602 ; RRID:Addgene_89602) -
For your References section:
Structure of the Bro1 domain protein BROX and functional analyses of the ALIX Bro1 domain in HIV-1 budding. Zhai Q, Landesman MB, Robinson H, Sundquist WI, Hill CP. PLoS One. 2011;6(12):e27466. doi: 10.1371/journal.pone.0027466. Epub 2011 Dec 5. 10.1371/journal.pone.0027466 PubMed 22162750
Map uploaded by the depositor.
