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Addgene

Lenti-dCas9-KRAB-blast
(Plasmid #89567)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89567 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    plenti
  • Total vector size (bp) 14229
  • Modifications to backbone
    replace VP64 with KRAB domain
  • Vector type
    Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Only amplify in recombinase deficient bacteria (eg. Stbl3 or HST08).
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9
  • Species
    Synthetic
  • Mutation
    D10A and H840A
  • Tags / Fusion Proteins
    • KRAB (C terminal on insert)
    • Blasticidin resistance gene (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lenti-dCas9-KRAB-blast was a gift from Gary Hon (Addgene plasmid # 89567 ; http://n2t.net/addgene:89567 ; RRID:Addgene_89567)

  • For your References section:

    Multiplexed Engineering and Analysis of Combinatorial Enhancer Activity in Single Cells. Xie S, Duan J, Li B, Zhou P, Hon GC. Mol Cell. 2017 Apr 20;66(2):285-299.e5. doi: 10.1016/j.molcel.2017.03.007. Epub 2017 Apr 13. 10.1016/j.molcel.2017.03.007 PubMed 28416141
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