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Addgene

tubulin-YeNL_pcDNA3
(Plasmid #89519)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89519 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YeNL
  • Species
    Synthetic
  • Insert Size (bp)
    1191
  • GenBank ID
    LC128716
  • Promoter CMV
  • Tag / Fusion Protein
    • b-tubulin (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGHrev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Derived from NanoLuc luciferase

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    tubulin-YeNL_pcDNA3 was a gift from Takeharu Nagai (Addgene plasmid # 89519 ; http://n2t.net/addgene:89519 ; RRID:Addgene_89519)

  • For your References section:

    Five colour variants of bright luminescent protein for real-time multicolour bioimaging. Suzuki K, Kimura T, Shinoda H, Bai G, Daniels MJ, Arai Y, Nakano M, Nagai T. Nat Commun. 2016 Dec 14;7:13718. doi: 10.1038/ncomms13718. 10.1038/ncomms13718 PubMed 27966527
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