VN-beta Synuclein
(Plasmid #89483)

• Purpose: expresses human beta Synuclein fused to the N-terminal part of Venus
• Depositing Lab: Tiago Outeiro
• Publication: Tenreiro et al Hum Mol Genet. 2016 Jan 15;25(2):275-90. doi: 10.1093/hmg/ddv470. Epub 2015 Nov 18.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5941
• Total vector size (bp): 6345
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SNCB
• Species: H. sapiens (human)
• Insert Size (bp): 404
• GenBank ID: XM_006714916
• Entrez Gene: SNCB
• Promoter: CMV
• Tag / Fusion Protein:
  • N-terminal part of Venus (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AflII (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: bGH

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Original clone (VN-alpha-Synuclein) comes from Pamela J.McLean.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Original clone (VN-alpha Synuclein) includes already the N-terminal part of venus, and was a gift from Pamela J.McLean.