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Addgene

pRSF-pLtetO-gfp
(Plasmid #89476)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89476 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSF
  • Backbone manufacturer
    Sergey V Mashko
  • Backbone size w/o insert (bp) 8728
  • Total vector size (bp) 9664
  • Modifications to backbone
    inserted pLtetO-gfpmut3b
  • Selectable markers
    Carbanicillin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pLtetO-GFPmut3b
  • Insert Size (bp)
    717
  • Promoter pLtetO

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GGCATCAAATTAAGCAGAAGGCCATCCTGA
  • 3′ sequencing primer GCTTCCCGTTTCAGCTGCGTTTC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2016/06/12/058487 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSF-pLtetO-gfp was a gift from George Church (Addgene plasmid # 89476 ; http://n2t.net/addgene:89476 ; RRID:Addgene_89476)

  • For your References section:

    Functional genomics of the rapidly replicating bacterium Vibrio natriegens by CRISPRi. Lee HH, Ostrov N, Wong BG, Gold MA, Khalil AS, Church GM. Nat Microbiol. 2019 Apr 8. pii: 10.1038/s41564-019-0423-8. doi: 10.1038/s41564-019-0423-8. 10.1038/s41564-019-0423-8 PubMed 30962569
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