coel::GFP
(Plasmid #8937)

• Depositing Lab: Piali Sengupta
• Publication: Miyabayashi et al Dev Biol. 1999 Nov 15. 215(2):314-31.

Backbone

• Vector backbone: pPD95.77
• Backbone size w/o insert (bp): 4500
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: unc-122 promoter
• Alt name: coel::GFP
• Alt name: unc-122
• Species: C. elegans (nematode)
• Insert Size (bp): 4000
• Entrez Gene: unc-122 (a.k.a. CELE_F11C3.2)
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PstI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: M13R

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Marker for expression of GFP in coelomocytes. 4kb unc-122 promoter+ 4aa of 1st exon (8911-12903 of cosmid F11C3, start is at 12892) in Pst-BamHI sites of pPD95.77.