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Addgene

pSG5-SUV39H2-FL
(Plasmid #89135)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89135 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSG5
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SUV39H2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3093
  • Entrez Gene
    SUV39H2 (a.k.a. KMT1B)
  • Promoter SV40

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSG5-SUV39H2-FL was a gift from Elizabeth Wilson (Addgene plasmid # 89135 ; http://n2t.net/addgene:89135 ; RRID:Addgene_89135)

  • For your References section:

    Androgen receptor regulation by histone methyltransferase Suppressor of variegation 3-9 homolog 2 and Melanoma antigen-A11. Askew EB, Bai S, Parris AB, Minges JT, Wilson EM. Mol Cell Endocrinol. 2017 Mar 5;443:42-51. doi: 10.1016/j.mce.2016.12.028. Epub 2016 Dec 29. 10.1016/j.mce.2016.12.028 PubMed 28042025
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