p5M-PR-A
(Plasmid #89124)

• Purpose: mammalian expression of full-length human progesterone receptor-A in pCMV5 with 3 restriction sites deleted in polylinker
• Depositing Lab: Elizabeth Wilson
• Publication: Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24. doi: 10.1074/jbc.M112.372797. Epub 2012 Aug 13.

Backbone

• Vector backbone: pCMV5
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human progesterone receptor-A
• Species: H. sapiens (human)
• Insert Size (bp): 2300
• Entrez Gene: PGR (a.k.a. NR3C3, PR)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene's sequencing results found that only 3 restriction sites from pCMV5 were deleted in this plasmid: HindIII, PstI, SalI.
BamHI and XbaI sites are still present in the polylinker.