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Addgene

VP-ARF
(Plasmid #89122)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89122 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pVP16-CT
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    p14-hARF
  • Alt name
    human ARF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    546
  • Entrez Gene
    CDKN2A (a.k.a. ARF, CAI2, CDK4I, CDKN2, CMM2, INK4, INK4A, MLM, MTS-1, MTS1, P14, P14ARF, P16, P16-INK4A, P16INK4, P16INK4A, P19, P19ARF, TP16)
  • Tag / Fusion Protein
    • VP16 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    VP-ARF was a gift from Elizabeth Wilson (Addgene plasmid # 89122 ; http://n2t.net/addgene:89122 ; RRID:Addgene_89122)

  • For your References section:

    Post-translational Down-regulation of Melanoma Antigen-A11 (MAGE-A11) by Human p14-ARF Tumor Suppressor. Minges JT, Grossman G, Zhang P, Kafri T, Wilson EM. J Biol Chem. 2015 Oct 9;290(41):25174-87. doi: 10.1074/jbc.M115.663641. Epub 2015 Sep 1. 10.1074/jbc.M115.663641 PubMed 26330556
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