pcDNA3HA-AR-LBD
(Plasmid #89111)

• Purpose: mammalian expression of human androgen receptor ligand binding domain amino acid residues 624-919 with N-terminal double HA tag
• Depositing Lab: Elizabeth Wilson
• Publication: He et al J Biol Chem. 1999 Dec 24;274(52):37219-25. doi: 10.1074/jbc.274.52.37219.

Backbone

• Vector backbone: pcDNA3-HA
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hAR ligand binding domain
• Species: H. sapiens (human)
• Insert Size (bp): 800
• Mutation: contains Ligand Binding Domain (624-919)
• Entrez Gene: AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
• Promoter: CMV
• Tag / Fusion Protein:
  • 2xHA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HpaI (destroyed during cloning)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry