GAL-AR-1-503
(Plasmid
#89093)
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Purposemammalian expression of human androgen receptor N-terminal 1-503 amino acids linked to GAL4 DNA binding domain
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 89093 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGALO
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namehAR aa 1-503
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SpeciesH. sapiens (human)
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Entrez GeneAR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
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Tag
/ Fusion Protein
- GAL4 DBD (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GAL-AR-1-503 was a gift from Elizabeth Wilson (Addgene plasmid # 89093 ; http://n2t.net/addgene:89093 ; RRID:Addgene_89093) -
For your References section:
Evidence for an anti-parallel orientation of the ligand-activated human androgen receptor dimer. Langley E, Zhou ZX, Wilson EM. J Biol Chem. 1995 Dec 15;270(50):29983-90. doi: 10.1074/jbc.270.50.29983. 10.1074/jbc.270.50.29983 PubMed 8530400