pCMV-hARdelta120-472
(Plasmid
#89088)
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Purposemammalian expression of human androgen receptor with deletion of 120-472 amino acid residues
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 89088 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV5
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehAR-120-472 N-term deletion
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SpeciesH. sapiens (human)
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MutationhAR-120-472
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Entrez GeneAR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-hARdelta120-472 was a gift from Elizabeth Wilson (Addgene plasmid # 89088 ; http://n2t.net/addgene:89088 ; RRID:Addgene_89088) -
For your References section:
Epidermal-growth-factor-dependent phosphorylation and ubiquitinylation of MAGE-11 regulates its interaction with the androgen receptor. Bai S, Wilson EM. Mol Cell Biol. 2008 Mar;28(6):1947-63. doi: 10.1128/MCB.01672-07. Epub 2008 Jan 22. 10.1128/MCB.01672-07 PubMed 18212060