pCMV-hARdeltaNH2-6
(Plasmid
#89104)
-
Purposemammalian expression of human androgen receptor amino acids 507-919
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 89104 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCMV5
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namehAR DNA binding domain+ligand binding domain
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1400
-
MutationNH2 terminal deletion, contains amino acids 507-919
-
Entrez GeneAR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCMV-hARdeltaNH2-6 was a gift from Elizabeth Wilson (Addgene plasmid # 89104 ; http://n2t.net/addgene:89104 ; RRID:Addgene_89104) -
For your References section:
Transcriptional activation and nuclear targeting signals of the human androgen receptor. Simental JA, Sar M, Lane MV, French FS, Wilson EM. J Biol Chem. 1991 Jan 5;266(1):510-8. PubMed 1985913