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Addgene

pCMV-hARdeltaNH2-6
(Plasmid #89104)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89104 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCMV5
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hAR DNA binding domain+ligand binding domain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1400
  • Mutation
    NH2 terminal deletion, contains amino acids 507-919
  • Entrez Gene
    AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-hARdeltaNH2-6 was a gift from Elizabeth Wilson (Addgene plasmid # 89104 ; http://n2t.net/addgene:89104 ; RRID:Addgene_89104)
  • For your References section:

    Transcriptional activation and nuclear targeting signals of the human androgen receptor. Simental JA, Sar M, Lane MV, French FS, Wilson EM. J Biol Chem. 1991 Jan 5;266(1):510-8. PubMed 1985913