pCMV-hAR1-660
(Plasmid #89079)

• Purpose: mammalian expression of human androgen receptor N-terminal fragment 1-660 amino acids
• Depositing Lab: Elizabeth Wilson
• Publication: Simental et al J Biol Chem. 1991 Jan 5;266(1):510-8.

Backbone

• Vector backbone: pCMV5
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hAR-1-660
• Species: H. sapiens (human)
• Mutation: aa 1-660 (contains exons A,B,C and part of D)
• Entrez Gene: AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: HindIII (destroyed during cloning)
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry