pBABE puro PPAR gamma 2
(Plasmid #8859)

• Depositing Lab: Bruce Spiegelman
• Publication: Tontonoz et al Cell. 1994 Dec 30. 79(7):1147-56.

Backbone

• Vector backbone: pBABE puro
• Backbone manufacturer: Available at Addgene (#1764)
• Backbone size w/o insert (bp): 5169
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PPAR gamma
• Alt name: PPAR gamma 2
• Alt name: PPARg2
• Species: M. musculus (mouse)
• Insert Size (bp): 2100
• GenBank ID: NM_011146
• Entrez Gene: Pparg (a.k.a. Nr1c3, PPAR-gamma, PPAR-gamma2, PPARgamma, PPARgamma2)
• Tags / Fusion Proteins:
  • Flag (N terminal on insert)
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pBABE5

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The PPARg2 cDNA was isolated from the adipocyte lambda ZAPII library derived from pBluescript-PPARg2 plasmid (Tontonoz et al 1994, Genes Dev 8:1224-1234) as an XbaI-XhoI fragment. Blunt end ligation into EcoRI site of pBABE puro.