pET26b-Granzyme A
(Plasmid #8823)

• Depositing Lab: Judy Lieberman
• Publication: Beresford et al Proc Natl Acad Sci U S A 1997 Aug 19;94(17):9285-90.

Backbone

• Vector backbone: pET26b(+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5360
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: For protein expression, use only BL21(DE3) competent cell from Novagen
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Human Granzyme A
• Species: H. sapiens (human)
• Insert Size (bp): 716
• Mutation: it mature enzyme.
• GenBank ID: NM_006144
• Entrez Gene: GZMA (a.k.a. CTLA3, HFSP)
• Tags / Fusion Proteins:
  • (His)6 (C terminal on backbone)
  • Enterokinase site (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: Xho I (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminator

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry