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PurposeGal4VP16 driven expression of NanoLuc
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 87696 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGL4.23
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNanoLuc
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SpeciesSynthetic
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Tag
/ Fusion Protein
- nuclear localization signal (C terminal on insert)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byThis vector plasmid was modified from pUAS-luc2 (Liqun Luo lab, Addgene plasmid # 24343).
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUAS-NanoLuc was a gift from Robert Campbell (Addgene plasmid # 87696 ; http://n2t.net/addgene:87696 ; RRID:Addgene_87696) -
For your References section:
Optogenetic control with a photocleavable protein, PhoCl. Zhang W, Lohman AW, Zhuravlova Y, Lu X, Wiens MD, Hoi H, Yaganoglu S, Mohr MA, Kitova EN, Klassen JS, Pantazis P, Thompson RJ, Campbell RE. Nat Methods. 2017 Mar 13. doi: 10.1038/nmeth.4222. 10.1038/nmeth.4222 PubMed 28288123
