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PurposeFor creating drosophila transgenics expressing UAS-3xHalo7::CAAX
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 87646 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJFRC81
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name3xHalo7::CAAX
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GenBank accession number: KY511556
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
UAS-3xHalo7::CAAX was a gift from Gregory Jefferis (Addgene plasmid # 87646 ; http://n2t.net/addgene:87646 ; RRID:Addgene_87646) -
For your References section:
Second-Generation Drosophila Chemical Tags: Sensitivity, Versatility, and Speed. Sutcliffe B, Ng J, Auer TO, Pasche M, Benton R, Jefferis GS, Cachero S. Genetics. 2017 Apr;205(4):1399-1408. doi: 10.1534/genetics.116.199281. Epub 2017 Feb 16. 10.1534/genetics.116.199281 PubMed 28209589