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Addgene

D5 gene in PETCON vector
(Plasmid #87626)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87626 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PCTCON2
  • Backbone manufacturer
    Wittrup lab
  • Backbone size w/o insert (bp) 6201
  • Total vector size (bp) 6975
  • Modifications to backbone
    none
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Designed allosteric Zn2+ and fluorescein binding antibody scFv D5
  • Species
    Synthetic
  • Insert Size (bp)
    774
  • Tags / Fusion Proteins
    • cMyc tag on backbone (C terminal on backbone)
    • 3x(G4S) Linker (N terminal on backbone)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer UPGS_fo : GGACAATAGCTCGACGATTGAAGGTAGATACCCATA
  • 3′ sequencing primer PCTCON_rev_2: CAGTGTAGATGTAACAAAATCGA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    D5 gene in PETCON vector was a gift from Sarel Fleishman (Addgene plasmid # 87626 ; http://n2t.net/addgene:87626 ; RRID:Addgene_87626)

  • For your References section:

    Incorporating an allosteric regulatory site in an antibody through backbone design. Khersonsky O, Fleishman SJ. Protein Sci. 2017 Apr;26(4):807-813. doi: 10.1002/pro.3126. Epub 2017 Mar 6. 10.1002/pro.3126 PubMed 28142198
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