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Addgene

XX>sgRNA F+E
(Plasmid #87543)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87543 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcesa

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XX>sgRNA F+E was a gift from Lionel Christiaen (Addgene plasmid # 87543 ; http://n2t.net/addgene:87543 ; RRID:Addgene_87543)

  • For your References section:

    Evaluation and rational design of guide RNAs for efficient CRISPR/Cas9-mediated mutagenesis in Ciona. Gandhi S, Haeussler M, Razy-Krajka F, Christiaen L, Stolfi A. Dev Biol. 2017 May 1;425(1):8-20. doi: 10.1016/j.ydbio.2017.03.003. Epub 2017 Mar 22. 10.1016/j.ydbio.2017.03.003 PubMed 28341547
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