Skip to main content
Addgene

3156 pBS Bad cDNA
(Plasmid #8752)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8752 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBluescript II SK(-)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BAD
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1100
  • GenBank ID
    NM_007522
  • Entrez Gene
    Bad (a.k.a. AI325008, Bbc2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Digestion with EcoRI will release bands of 700bp and 400bp.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3156 pBS Bad cDNA was a gift from Stanley Korsmeyer (Addgene plasmid # 8752 ; http://n2t.net/addgene:8752 ; RRID:Addgene_8752)

  • For your References section:

    Bad, a heterodimeric partner for Bcl-XL and Bcl-2, displaces Bax and promotes cell death. Yang E, Zha J, Jockel J, Boise LH, Thompson CB, Korsmeyer SJ. Cell 1995 Jan 27;80(2):285-91. 10.1016/0092-8674(95)90411-5 PubMed 7834748
Related items:
Commonly requested with: