pCG-D-SpyTag-His6-E
(Plasmid
#87375)
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PurposeAn ORF part-containing vector harbouring the SpyTag sequence with a His6 tag at its C-terminus and 4 bp overhangs specific for the ‘downstream SpyPart’ position within an ORF assembly
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 87375 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepYTK001
- Backbone size w/o insert (bp) 2676
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSpyTag
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Tag
/ Fusion Protein
- His6 tag (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCG-D-SpyTag-His6-E was a gift from Tom Ellis (Addgene plasmid # 87375 ; http://n2t.net/addgene:87375 ; RRID:Addgene_87375) -
For your References section:
Extracellular Self-Assembly of Functional and Tunable Protein Conjugates from Bacillus subtilis. Gilbert C, Howarth M, Harwood CR, Ellis T. ACS Synth Biol. 2017 Mar 7. doi: 10.1021/acssynbio.6b00292. 10.1021/acssynbio.6b00292 PubMed 28230977
