C321.∆A.opt
(Bacterial strain #87359)

• Purpose: This is C321.∆A with additional mutations engineered by Kuznetsov et al. to improve doubling time.
• Depositing Lab: George Church
• Publication: Kuznetsov et al Genome Biol. 2017 May 25;18(1):100. doi: 10.1186/s13059-017-1217-z.

Backbone

• Vector backbone: Whole genome
• Vector type: Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): C321.∆A.opt strain
• Growth instructions: Do not grow above 34 C.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: C321.∆A.opt strain
• Species: Synthetic

Resource Information

• Supplemental Documents:
  • c321.DA.opt. MAGE oligos and MASC-PCR primers
  • allele-specific PCR
  • Image of PCR verification
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

C321.∆A.opt is a variant of C321.∆A (Addgene Item #48998) engineered to have a faster growth rate, as reported in Kuznetsov, Gleb, et al. "Optimizing complex phenotypes through model-guided multiplex genome engineering." bioRxiv (2016): 086595.

This strain has a heat-inducible lambda prophage (42 C) and should be grown at or below 34 C to avoid induction.

The original strain C321.∆A (Addgene Item #48998) was reported in Lajoie, Marc J., et al. "Genomically recoded organisms expand biological functions." Science 342.6156 (2013): 357-360.