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Addgene

m.Setd7
(Plasmid #87131)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87131 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+ MycTag
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Setd7
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Setd7 (a.k.a. 1600028F23Rik, H3K4MT, KMT7, Set7, Set7/9, mKIAA1717)
  • Promoter simian CMV IE94
  • Tag / Fusion Protein
    • 6x-Myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    m.Setd7 was a gift from Francesca Spagnoli (Addgene plasmid # 87131 ; http://n2t.net/addgene:87131 ; RRID:Addgene_87131)

  • For your References section:

    The histone methyltransferase Setd7 promotes pancreatic progenitor identity. Kofent J, Zhang J, Spagnoli FM. Development. 2016 Oct 1;143(19):3573-3581. Epub 2016 Aug 30. 10.1242/dev.136226 PubMed 27578787
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