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pCDH-Flag-mPRMT7-GFP
(Plasmid #87104)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87104 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDH-EF1-IRES-GFP
  • Backbone manufacturer
    System Biosciences
  • Backbone size w/o insert (bp) 7200
  • Total vector size (bp) 9300
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Protein arginine methyltransferase 7
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2103
  • Entrez Gene
    Prmt7 (a.k.a. 4933402B05Rik, BC006705)
  • Promoter EF1
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site BglII (destroyed during cloning)
  • 5′ sequencing primer EF1 sequencing primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDH-Flag-mPRMT7-GFP was a gift from Min Gyu Lee (Addgene plasmid # 87104 ; http://n2t.net/addgene:87104 ; RRID:Addgene_87104)

  • For your References section:

    A feedback loop comprising PRMT7 and miR-24-2 interplays with Oct4, Nanog, Klf4 and c-Myc to regulate stemness. Lee SH, Chen TY, Dhar SS, Gu B, Chen K, Kim YZ, Li W, Lee MG. Nucleic Acids Res. 2016 Dec 15;44(22):10603-10618. Epub 2016 Sep 12. 10.1093/nar/gkw788 PubMed 27625395
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