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pET28b-pchA WT
(Plasmid #87102)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87102 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b
  • Backbone manufacturer
    novagen
  • Backbone size w/o insert (bp) 5368
  • Total vector size (bp) 6799
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    Room Temperature
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Grown in terrific broth with 0.4% glycerol for 22 hours after induction.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pchA WT
  • Species
    Pseudomonas aeruginosa PAO1
  • Insert Size (bp)
    1431
  • Promoter T7
  • Tag / Fusion Protein
    • his-tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pET upstream
  • 3′ sequencing primer T7 reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Archives of Biochemistry and Biophysics, 539 (2013) 70-80, PMC3836504
JACS 138 (2016) 9277-9293, PMC 5029964

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28b-pchA WT was a gift from Audrey Lamb (Addgene plasmid # 87102 ; http://n2t.net/addgene:87102 ; RRID:Addgene_87102)

  • For your References section:

    Lysine221 is the general base residue of the isochorismate synthase from Pseudomonas aeruginosa (PchA) in a reaction that is diffusion limited. Meneely KM, Luo Q, Dhar P, Lamb AL. Arch Biochem Biophys. 2013 Oct 1;538(1):49-56. doi: 10.1016/j.abb.2013.07.026. Epub 2013 Aug 11. 10.1016/j.abb.2013.07.026 PubMed 23942051
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