Stat3 pcDNA3
(Plasmid #8706)

• Purpose: See Depositor Comments below for variant information.
• Depositing Lab: Jim Darnell
• Publication: Zhong et al Proc Natl Acad Sci U S A 1994 May 24;91(11):4806-10.

Backbone

• Vector backbone: pcDNA3.0
• Backbone manufacturer: Identical to pRc/CMV except at its MCS
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Stat3
• Species: M. musculus (mouse)
• Insert Size (bp): 2400
• Mutation: * see comments
• GenBank ID: U06922
• Entrez Gene: Stat3 (a.k.a. 1110034C02Rik, Aprf)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Diagnostic restriction digest with XmaI (1.6kb and 6.1kb).

* E16K mutation found during QC. This variant is found in other published versions of this protein; should function as described in associated publication.