pDUAL OCLN (GFP)
(Plasmid
#86982)
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PurposeLentiviral expression construct encoding Occludin and GFP from separate promoters
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86982 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDUAL GFP
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOCLN
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Alt nameNP_002529.1
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SpeciesH. sapiens (human)
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Entrez GeneOCLN (a.k.a. BLCPMG, PPP1R115, PTORCH1)
- Promoter SFFV/hPGK
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer SFFVpro-F (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDUAL OCLN (GFP) was a gift from Joe Grove (Addgene plasmid # 86982 ; http://n2t.net/addgene:86982 ; RRID:Addgene_86982)
