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PurposeLentiviral expression construct encoding SR-B1 and GFP from separate promoters
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 86979 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDUAL GFP
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSCARB1
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Alt nameCAA80277.1
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SpeciesH. sapiens (human)
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Entrez GeneSCARB1 (a.k.a. CD36L1, CLA-1, CLA1, HDLCQ6, HDLQTL6, SR-BI, SRB1)
- Promoter SFFV/hPGK
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer SFFVpro-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Depositor notes that S97F mutation in SCARB1 insert does not affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDUAL SRBI (GFP) was a gift from Joe Grove (Addgene plasmid # 86979 ; http://n2t.net/addgene:86979 ; RRID:Addgene_86979)