pRAF-MutS
(Plasmid
#86605)
-
PurposeE. coli MutS expression plasmid
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86605 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepET29a
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 7900
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameE. coli MutS (D835R,R840E)
-
SpeciesE. coli
-
Insert Size (bp)2661
-
MutationD835R, R840E
-
GenBank IDNP_417213.1
- Promoter T7
-
Tag
/ Fusion Protein
- his-tag and ald-tag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pRAF-MutS was a gift from Richard Fishel (Addgene plasmid # 86605 ; http://n2t.net/addgene:86605 ; RRID:Addgene_86605) -
For your References section:
Cascading MutS and MutL sliding clamps control DNA diffusion to activate mismatch repair. Liu J, Hanne J, Britton BM, Bennett J, Kim D, Lee JB, Fishel R. Nature. 2016 Nov 16;539(7630):583-587. doi: 10.1038/nature20562. 10.1038/nature20562 PubMed 27851738
