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CRiNCL - Human CRISPRi Non-coding Libraries
(Pooled Libraries #86538, #86539, #86540, #86541, #86542, #86543, #86544, #86545, #86546, #86547, #86548, #86549, #86550)

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  • Purpose

    These CRISPRi subpooled libraries (10 gRNAs/TSS) are designed to target long non-coding RNA (lncRNA)-expressing genes rather than coding genes in order to discover non-coding genes with cellular functions.

  • Vector Backbone

    pCRISPRia-v2 (Plasmid #84832) - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 86538 CRISPRi non-coding library (CRiNCL) - Common to all 7 cell lines† 1 $640 Add to Cart
Pooled Library 86539 CRISPRi non-coding library (CRiNCL) - Common to all 5 cancer cell lines† 1 $325 Add to Cart
Pooled Library 86540 CRISPRi non-coding library (CRiNCL) - Unique to iPSC† 1 $325 Add to Cart
Pooled Library 86541 CRISPRi non-coding library (CRiNCL) - Unique to HFF† 1 $325 Add to Cart
Pooled Library 86542 CRISPRi non-coding library (CRiNCL) - Unique to U87† 1 $325 Add to Cart
Pooled Library 86543 CRISPRi non-coding library (CRiNCL) - Unique to HEK293T† 1 $325 Add to Cart
Pooled Library 86544 CRISPRi non-coding library (CRiNCL) - Unique to K562† 1 $325 Add to Cart
Pooled Library 86545 CRISPRi non-coding library (CRiNCL) - Unique to HeLa† 1 $325 Add to Cart
Pooled Library 86546 CRISPRi non-coding library (CRiNCL) - Unique to MCF7† 1 $325 Add to Cart
Pooled Library 86547 CRISPRi non-coding library (CRiNCL) - U87 & HEK293T† 1 $325 Add to Cart
Pooled Library 86548 CRISPRi non-coding library (CRiNCL) - K562, HeLa, & MCF7† 1 $325 Add to Cart
Pooled Library 86549 CRISPRi non-coding library (CRiNCL) - K562 & HeLa† 1 $325 Add to Cart
Pooled Library 86550 CRISPRi non-coding library (CRiNCL) - iPSC & HFF† 1 $325 Add to Cart
Available to academic and nonprofits only.
†A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used with dCas9-KRAB-BFP (#46911) or otherwise used with cell lines already expressing inhibitory dCas9.

If you would like to receive all thirteen sublibraries in a single order, please email [email protected] for pricing (if you use a distributor in your country, please contact your distributor directly for pricing).

Library Details

Pooled Library Genes Targeted gRNAs Control gRNAs
86538 1,329 13,548 258
86539 675 6,903 153
86540 2,965 29,976 326
86541 4,103 41,511 481
86542 2,307 23,317 247
86543 2,253 22,869 339
86544 2,831 28,501 191
86545 2,337 23,693 323
86546 2,913 29,617 487
86547 1,443 14,694 264
86548 875 8,944 194
86549 1,051 10,794 284
86550 1,269 12,950 260
  • Species
    Human
  • Lentiviral Generation
    3rd

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL

Resource Information

Depositor Comments

The data analysis pipeline (Link opens in a new window) for analyzing sequencing data is available on GitHub.

A schematic of sublibrary divisions of the CRiNCL library. The "Common to all 7 cell lines" sublibrary targets 1,329 genes. The iPSC and HFF sublibrary targets 1,269 genes. The unique to iPSC sublibrary targets 2,965. The unique to HFF sublibrary targets 4,103. The "Common to all 5 cancer cell lines" sublibrary targets 675 genes. The U87 and HEK293FT sublibrary targets 1,443 genes. The K562, HeLa, and MCF7 sublibrary targets 875 genes. The unique to U87 sublibrary targets 2,307 genes. The unique to HEK293FT library targets 2,253 genes. The K562 and HeLa sublibrary targets 1,051 genes. The unique to K562 sublibrary targets 2,831 genes. The unique to HeLa sublibrary targets 2,337 genes. The unique to MCF7 sublibrary target 2,913 genes.

Figure 1: Schematic of CRiNCL sublibraries

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human CRISPRi non-coding library (CRinCL) was a gift from Jonathan Weissman (Addgene #86538)
    Human CRISPRi non-coding library (CRinCL) was a gift from Jonathan Weissman (Addgene #86539)
    Human CRISPRi non-coding library (CRinCL) was a gift from Jonathan Weissman (Addgene #86540)
    Human CRISPRi non-coding library (CRinCL) was a gift from Jonathan Weissman (Addgene #86541)
    Human CRISPRi non-coding library (CRinCL) was a gift from Jonathan Weissman (Addgene #86542)
    Human CRISPRi non-coding library (CRinCL) was a gift from Jonathan Weissman (Addgene #86543)
    Human CRISPRi non-coding library (CRinCL) was a gift from Jonathan Weissman (Addgene #86544)
    Human CRISPRi non-coding library (CRinCL) was a gift from Jonathan Weissman (Addgene #86545)
    Human CRISPRi non-coding library (CRinCL) was a gift from Jonathan Weissman (Addgene #86546)
    Human CRISPRi non-coding library (CRinCL) was a gift from Jonathan Weissman (Addgene #86547)
    Human CRISPRi non-coding library (CRinCL) was a gift from Jonathan Weissman (Addgene #86548)
    Human CRISPRi non-coding library (CRinCL) was a gift from Jonathan Weissman (Addgene #86549)
    Human CRISPRi non-coding library (CRinCL) was a gift from Jonathan Weissman (Addgene #86550)

  • For your References section:

    CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells. Liu SJ, Horlbeck MA, Cho SW, Birk HS, Malatesta M, He D, Attenello FJ, Villalta JE, Cho MY, Chen Y, Mandegar MA, Olvera MP, Gilbert LA, Conklin BR, Chang HY, Weissman JS, Lim DA. Science. 2017 Jan 06. Vol. 355, Issue 6320. pii: aah7111. PubMed 27980086
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