UBXD1-GFP
(Plasmid #86464)

• Purpose: Expression of human UBXD1 with C-terminal GFP tag
• Depositing Lab: Hemmo Meyer
• Publication: Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Backbone

• Vector backbone: pEGFP-N1
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: UBXD1
• Alt name: UBXN6
• Alt name: UBXDC2
• Species: H. sapiens (human)
• GenBank ID: NP_079517
• Entrez Gene: UBXN6 (a.k.a. UBXD1, UBXDC2)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: pEGFP-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

cloned from pIRESpuro2-UBXD1-3xHA (#31836)