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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8640 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1 (+)
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 6353
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIntegrin beta 2
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Alt nameCD18
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Alt namepCDB1
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SpeciesH. sapiens (human)
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GenBank IDNM_000211
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Entrez GeneITGB2 (a.k.a. CD18, LAD, LCAMB, LFA-1, MAC-1, MF17, MFI7)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Human Integrin beta 2 was a gift from Timothy Springer (Addgene plasmid # 8640 ; http://n2t.net/addgene:8640 ; RRID:Addgene_8640) -
For your References section:
Cloning of the beta subunit of the leukocyte adhesion proteins: homology to an extracellular matrix receptor defines a novel supergene family. Kishimoto TK, O'Connor K, Lee A, Roberts TM, Springer TA. Cell 1987 Feb 27;48(4):681-90. 10.1016/0092-8674(87)90246-7 PubMed 3028646